ms2v6 stem Search Results


ms2v6 stem  (Addgene inc)
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Addgene inc ms2v6 stem
Design principles of SunRISER are generalizable to orthogonal stem loops and protein-tagging systems (A) HeLa cells transfected with detection plasmids phage-cmv-CFP-24×MS2 (top) and <t>phage-cmv-CFP-24×MS2V6</t> (bottom) stem-loop variants of SunRISER with ubc-nls-MCP-5×SunTag show similar characteristics and intensity distributions, quantified in histograms (right, top: n = 30 for cell numbers and n = 8,227 for spots numbers; bottom: n = 21 for cell numbers and n = 4,627 for spots numbers). (B) HeLa cells transfected with detection plasmid phage-cmv-cfp-24×pp7 with cmv-sfGFP-GB1-Nb-gp41 and ubc-nls-PCP-12×MoonTag, quantified in histograms (right, n = 19 for cell numbers and n = 4,534 for spots numbers). We note that the MoonRISER example can be further optimized as it uses a longer 12× MoonTag and a nanobody that has different binding properties. Cells were imaged with 60× wide-field microscope 24 h after transfection and quantified with dNEMO ( <xref ref-type=Kowalczyk et al., 2021 ) Scale bar: 10 μm. See also Figure S5 . " width="250" height="auto" />
Ms2v6 Stem, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ms2v6 stem - by Bioz Stars, 2026-04
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Design principles of SunRISER are generalizable to orthogonal stem loops and protein-tagging systems (A) HeLa cells transfected with detection plasmids phage-cmv-CFP-24×MS2 (top) and phage-cmv-CFP-24×MS2V6 (bottom) stem-loop variants of SunRISER with ubc-nls-MCP-5×SunTag show similar characteristics and intensity distributions, quantified in histograms (right, top: n = 30 for cell numbers and n = 8,227 for spots numbers; bottom: n = 21 for cell numbers and n = 4,627 for spots numbers). (B) HeLa cells transfected with detection plasmid phage-cmv-cfp-24×pp7 with cmv-sfGFP-GB1-Nb-gp41 and ubc-nls-PCP-12×MoonTag, quantified in histograms (right, n = 19 for cell numbers and n = 4,534 for spots numbers). We note that the MoonRISER example can be further optimized as it uses a longer 12× MoonTag and a nanobody that has different binding properties. Cells were imaged with 60× wide-field microscope 24 h after transfection and quantified with dNEMO ( <xref ref-type=Kowalczyk et al., 2021 ) Scale bar: 10 μm. See also Figure S5 . " width="100%" height="100%">

Journal: Cell Reports Methods

Article Title: Long-term imaging of individual mRNA molecules in living cells

doi: 10.1016/j.crmeth.2022.100226

Figure Lengend Snippet: Design principles of SunRISER are generalizable to orthogonal stem loops and protein-tagging systems (A) HeLa cells transfected with detection plasmids phage-cmv-CFP-24×MS2 (top) and phage-cmv-CFP-24×MS2V6 (bottom) stem-loop variants of SunRISER with ubc-nls-MCP-5×SunTag show similar characteristics and intensity distributions, quantified in histograms (right, top: n = 30 for cell numbers and n = 8,227 for spots numbers; bottom: n = 21 for cell numbers and n = 4,627 for spots numbers). (B) HeLa cells transfected with detection plasmid phage-cmv-cfp-24×pp7 with cmv-sfGFP-GB1-Nb-gp41 and ubc-nls-PCP-12×MoonTag, quantified in histograms (right, n = 19 for cell numbers and n = 4,534 for spots numbers). We note that the MoonRISER example can be further optimized as it uses a longer 12× MoonTag and a nanobody that has different binding properties. Cells were imaged with 60× wide-field microscope 24 h after transfection and quantified with dNEMO ( Kowalczyk et al., 2021 ) Scale bar: 10 μm. See also Figure S5 .

Article Snippet: 24×MS2V6 stem-loops from pET259-pUC57-24×MS2V6 (Addgene #104391 ( );) was amplified using BamHI and SacII sites to label cfp the same way as other two stem-loops.

Techniques: Transfection, Plasmid Preparation, Binding Assay, Microscopy

Journal: Cell Reports Methods

Article Title: Long-term imaging of individual mRNA molecules in living cells

doi: 10.1016/j.crmeth.2022.100226

Figure Lengend Snippet:

Article Snippet: 24×MS2V6 stem-loops from pET259-pUC57-24×MS2V6 (Addgene #104391 ( );) was amplified using BamHI and SacII sites to label cfp the same way as other two stem-loops.

Techniques: Recombinant, Software